Vitrification of biopsied embryos at cleavage, morula and blastocyst stage
نویسندگان
چکیده
منابع مشابه
Cryopreservation of biopsied embryos at the blastocyst stage.
BACKGROUND The availability of an efficient cryopreservation program is especially important in the case of embryos that have undergone blastomere biopsy for PGD. Unfortunately, the freezing/thawing of biopsied embryos has given disappointing results when performed at the cleavage stage. In this study, embryos diagnosed as normal after PGD were grown to the blastocyst stage, frozen and thawed f...
متن کاملClosed blastocyst vitrification of biopsied embryos: evaluation of 100 consecutive warming cycles.
BACKGROUND The aim of this study was to analyse the outcome of closed blastocyst vitrification of embryos biopsied at the cleavage stage. METHODS Vitrification of supernumerary blastocysts was performed using the closed CBS-VIT High Security straws. Warming cycles (n = 100) for patients with preimplantation genetic diagnosis (PGD) and/or aneuploidy screening in the fresh cycle were analysed. ...
متن کاملThe Effect of Biopsy During Precompacted Morula Stage on Post Vitrification Development of Blastocyst Derived Bovine Embryos
Improvements on embryo micromanipulation techniques led to the use of embryo biopsy in commercial embryo transfer programs for genetic analysis of preimplantation bovine embryos. The aim of this study was to evaluate the quality of bovine blastocyst derived from embryos biopsied at different pre-compacted morulae stages by assessment of cryosurvivability of the resulting blastocysts. The in vit...
متن کاملApplication of hollow fiber vitrification for cryopreservation of bovine early cleavage stage embryos and porcine morula-blastomeres
A novel hollow fiber vitrification (HFV) method was applied to materials that have previously been difficult to cryopreserve, thereby expanding the potential application of this method. The results showed that zona-free porcine morulae and their isolated blastomeres remained viable even after vitrification. The rate of development to blastocysts after vitrification was similar for zona-free and...
متن کاملMorphological and cytogenetic assessment of cleavage and blastocyst stage embryos.
Morphological assessments are the main way in which fertility clinics select in vitro generated embryo(s) for transfer to the uterus. However, it is widely acknowledged that the microscopic appearance of an embryo is only weakly correlated with its viability. Furthermore, the extent to which morphology is affected by aneuploidy, a genetic defect common in human preimplantation embryos, remains ...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2009
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2009.05.009